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We hope you enjoy the options presented! The introduction of the minor groove binder moiety is sequence-independent, too. In summary, metabion offers all three duplex stability enhancing modifications, and therefore provides greatest flexibility in assay design and choosing the right option for your required application.

Alternatively orders can be placed by sending an e-mail with our pre-formatted Excel order file as attachment. When designing LNA-containing oligonucleotides, one should follow the basic rules of primer design particularly pay attention to the location and number of LNAs.

Also, try to avoid stretches of more than 4 consecutive LNAs, which would result in very tight hybridization in that region. Avoid self-complementarity and cross-hybridization to other LNA-containing oligonucleotides. A concentration of 0.